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GFP and mCherry are fluorescent proteins

On the Galapagos islands, wild goats were a major problem. In less than five years, scientists wiped out tens of thousands with sterile Hari females. Biologist Karl Campbell of the non profit Island Conservation introduced specialized female goats that researchers sterilized and chemically altered into a permanent state of heat, to lure the male goats into fruitless goat sex.

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canada goose By mixing and matching all combinations of the four crRNAs with the four taRNAs, they confirmed that taRNAs are highly specific to their partner crRNA (often called “orthogonal,”) although the most highly mutated riboregulator had about a third of the activity as the others. With their toolkit now ready, they assembled a switchboard with four commonly used easily measured GFP, mCherry (a variant of RFP), LacZ, and firefly luciferase. GFP and mCherry are fluorescent proteins, which are measured by exciting the sample with a particular wavelength of light and measuring the light emitted at a different wavelength. canada goose

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